\(\textit{In vitro}\) shoot multiplication and the content of huperzine a in in vitro cultured \(\textit{Huperzia serrata}\) (Thunb. Ex Murray) Trevis plants
نویسندگان
چکیده
Huperzia serrata (Thunb. Ex Murray) Trevis belonging to the Lycopodiaceae family contains main active ingredient Huperzine A (HupA), which is effective in treatment of dementia. However, H. nature a low content HupA. Additionally, this plant grows very slowly and overexploited, thereby leading risk extinction. In study, we used vitro shoot induce multiplication by tissue culture method quantify HupA cultured High-Performance Liquid Chromatography (HPLC). The suitable nutrient medium for inducing was 1/4 MS supplemented with 1 mg/L kinetin. medium, rate as high 77.33%, number shoots/shoot 6.02, height 1.61 cm. Based on HPLC method, 300 µg/g. Vietnam reported first time our study. This study shows promising propagating can produce These findings contribute conserving preserving gene source species, at same time, enhancing serrata.
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ژورنال
عنوان ژورنال: Academia Journal of Biololy
سال: 2022
ISSN: ['2615-9023']
DOI: https://doi.org/10.15625/2615-9023/16207